![]() A homing endonuclease gene drive (HEG) works by deliberately breaking a recipient allele at the right spot (through the expression of the Cas9 protein and a guide RNA), set up for the donor allele to copy over the whole deliberate-break-it Cas9/guide RNA machinery sequence into the broken recipient chromosome. These take advantage of the double-strand-break DNA repair mechanism where a chromosome gets repaired by copying the sequence from a homologous chromosme - you end up with a stretch that’s no longer heterozygous (it’s now homozygous for whatever the donor strand sequence is), but at least it works, as opposed to being broken. The most straightforward of these would be a “ homing endonuclease” gene drive. The advent of CRISPR-Cas9 technology has really brought a lot more attention to these ideas, because they make them far more possible, for better or worse. There are a lot of potential mechanisms for this (see that link for a good review), but what you don’t see are the total-wipeout forms just mentioned, which is what we has humans might like to do to (say) mosquitos or tsetse flies. These are the so-called “selfish genes”, and some of these have no fitness advantage (or even some disadvantage) in the organisms themselves. Gene drives of various kinds show up in nature, though, when a gene has some sort of ability to control its own transmission. In fact, a number of gene drive ideas have the opposite sign, conferring catastrophic unfitness in order to wipe out pathogens and disease-vector organisms. This is a thumb-on-the-scale unnatural selection if ever there was one, because that biased inheritance is outside of any fitness advantage that the new sequence might bring with it. If none of the solutions works, you can use the option to upload your results to Microsoft, or go to Microsoft Community to request help.Consider the “gene drive” idea - there are a lot of variations, but the general idea is that you introduce a genetic sequence into an organism that can bias (drive) its own inheritance into the next generation. SaRA will run a set of diagnostic checks, and then return some possible solutions for you to try to fix Outlook connectivity issues. Install the Support and Recovery Assistant (SaRA) if prompted. It also gives you the option to upload your results to Microsoft so that a support engineer can review them. This step creates detailed information about your Outlook configuration and provides solutions for any known issues that are detected. Run the recovery assistant if you've tried all of the preceding steps and Outlook is still crashing or hanging. ![]() Run the Microsoft Support and Recovery Assistant When prompted, enter your sign in credentials, and then select OK.Ĥ. You might see the message Allow this website to configure server settings? Select the Don't ask me about this website again checkbox, and then select Allow. Type a name for the new profile in the Profile Name box.Įnter the user name and the primary SMTP address. If you're not sure whether the data files are backed up or stored on a server, do not remove the profile. Note: Removing a profile also removes any associated data files. Select the profile that you want to remove, and then select Remove. In the list of installed programs, right-click the entry for your Microsoft 365 installation and then select Change > Online Repair. Select Uninstall a program from the Programs group. Type Control Panel in the search box at the bottom of the Windows screen. If the issue recurs, remove the last add-in to be added. ![]() Repeat until either all add-ins have been re-added, or the issue recurs. ![]() If the issue doesn't recur, add the next add-in. If the issue doesn't recur, start adding the add-ins back one at a time.Īfter adding each add-in, exit then re-open Outlook. Select any active check boxes to clear them, and then select OK. If Outlook doesn't crash or hang, continue by selecting File > Options > Add-Ins.Īt the bottom of the window, select COM Add-ins and then select Go. Press the Windows logo key + R to open a Run dialog box.Įnter Outlook /safe, choose a profile if prompted, and then select OK. Investigate possible issues caused by add-ins If you go through all of those steps and Outlook is still crashing or not responding, complete step 4 before contacting the Microsoft Community for further help. Try steps 1 through 3 in order until the issue is cleared. Outlook crashes even though you aren't actively using it. This article describes how to troubleshoot Microsoft Outlook when it's used together with Microsoft 365 and: ![]()
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